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Sequence Alignment in a High-Throughput Way

$250-750 USD

Completed
Posted 5 months ago

$250-750 USD

Paid on delivery
I am looking for a freelancer who can help with sequence alignment in a high-throughput way. The desired output of the sequence alignment is a list of aligned sequences. Here is the description: We have an excel spreadsheet with tens of thousands of test DNA sequences. We need to compare them to another DNA sequence (benchmark) and select test sequences that have a short sequence (15-18 bp) of high identity within them A more detailed view: - benchmark sequence. It is just one sequence for each project. Its length can wary from 300bp to 5000bp - Tested sequences are all different. typically 30-50 bp, up to 100pb - need to identify test sequences that have at least one stretch of 15-18 bp with at least 50% identity to the benchmark Ideally, we would like to have a system that we can use ourselves in our lab but we are open for a service as well. Please let me know if that is something you can help us with. The project has a test file attached. Please contact me only if you can align at least 1 out of 3 test sequences and the benchmark sequence. We can discuss the real data after I make sure that you can do it.
Project ID: 37569880

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20 proposals
Remote project
Active 5 mos ago

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Hi there! I'm a freelancer with expertise in bioinformatics. Msc degree in Bioinformaticsand PhD in Genomics. I specialize in Next-Generation Sequencing (NGS) data analysis, Single Nucleotide Polymorphisms (SNPs) data analysis, Genome-Wide Association Studies (GWAS), and Enrichment Analysis. I believe I am the perfect person for the job! My technical skills include Python programming, R scripting and Bash scripting which allow me to provide comprehensive solutions to complex problems. Additionally, my commitment to quality ensures that all work is carried out to highest standards. I can provide you full pipeline you can automatically use it with full description. Please contact me for more information so i can give you examples from my github profile.
$500 USD in 7 days
5.0 (3 reviews)
3.0
3.0
20 freelancers are bidding on average $456 USD for this job
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Hi there! As I have read, I see you need help comparing tests and selecting similar sequences optimally and I could help you! I'm a professional Database Administrator and Analytics Engineer with more than 10 years of experience and more than 300 projects done here on this platform. I offer unlimited revisions and I don't require any payment in front. I always meet delivery deadlines working until I get the desired results. I know all the most used softwares to do this project specifically, I could even give you some other options in case you think is better to use other tools. Send me a message so I can show you my previous work and we can discuss more. Best regards, Giovanni.
$250 USD in 1 day
4.9 (26 reviews)
6.3
6.3
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Hello there, I will help with project on Sequence Alignment in a High-Throughput Way.I have read the instructions carefully and I clearly understand what is required of the project. I have handled several assignments on this field and I will be glad to offer my expertise and on this one too. With me, the quality and the deadline of your paper are my utmost priority. I have expertise needed to complete your project as i have skills in Bioinformatics and Data Processing. Feel free to contact me for more details.
$270 USD in 1 day
4.8 (17 reviews)
5.1
5.1
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hey, generally it's about running BLAST. the benchmark sequence would be the query and test sequences would be our database. but the question is not clear. I read the document and it's not clear for me why that example is a kind of wrong alignment. tell me please what exactly is the input? is that a benchmark sequence with underlined regions or simply benchmark sequence is provided without any underlined regions? you know they are two different stories. if you already underlined one or two regions, the best solution is to split those regions and running BLAST on those specific regions otherwise BLAST must be run on all the benchmark sequence. make it clear please. I would be available if you wanna discuss.
$400 USD in 3 days
5.0 (1 review)
4.6
4.6
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Hello! I've carefully checked your requirements and really interested in this project Sequence Alignment in a High-Throughput Way. I'm a full-time data and statistical analyst. I have worked with many clients across the world. I have rich experience in Data Processing and Bioinformatics. For more information about me, please refer to my portfolios on https://www.freelancer.com/hireme/monicawriter99. I'm ready to discuss your project and start immediately. Thank you. Monicah Writer.
$360 USD in 3 days
4.6 (10 reviews)
4.6
4.6
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Hi,i can deliver you excellent wrk i am Specialist on Bioinformatic and R langugae I have more than 10 years of experience in Data Processing and Alignment. My skillset includes Python, C++, HTML, CSS, R language and machine learning. I am confident that I can provide the necessary support for sequence alignment in a high-throughput way. Specifically, I possess the expertise needed to analyze large sets of test DNA sequences and select those that have a short sequence (15-18 bp) of high identity within them that need to be compared against a benchmark sequence. Additionally, I have developed systems that enable collaborative workflows between multiple team members. This makes it easier for me to implement your project requirements as well as adapt the solution to meet changing needs over time. I would love to discuss this project further with you if this sounds like something you might be interested in! Please let me know if you would like me to send more information about my services or just answer any other questions you may have about the project.
$300 USD in 7 days
5.0 (2 reviews)
2.8
2.8
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Hello, Good afternoon? I hope this proposal finds you well. As an experienced freelancer, I have honed my skills in Bioinformatics and Data Processing and am confident in delivering top-quality work. If you could kindly message me, we can discuss and get started soon. Much obliged
$555 USD in 1 day
5.0 (1 review)
0.6
0.6
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Hello, I have written a program for the attached test file and I have successfully aligned 3 test sequences with the benchmark with more than 50% identity. I can send you the results once you contact me as I am not allowed to attach any file with my proposal. Results: Test Sequence 1: Benchmark Position: 78 to 93 Test 1 Position: 26 to 41 Bases within identified stretch (Benchmark): GCCGCGATGACGGAGA Bases within identified stretch (Test): AGCGCGATGAAGGTGA Match percentage: 75.0% Test Sequence 2: Benchmark Position: 199 to 215 Test 2 Position: 66 to 82 Bases within identified stretch (Benchmark): CAGTGATAAGATATGTG Bases within identified stretch (Test): CAGTGtTAAGAaATGTG Match percentage: 88.23529411764706% Test Sequence 3: Benchmark Position: 199 to 215 Test 3 Position: 66 to 82 Bases within identified stretch (Benchmark): CAGTGATAAGATATGTG Bases within identified stretch (Test): CAGTGtTAAGAaATGTG Match percentage: 88.23529411764706% test sequence 2 and 3 find the macth in the underlined area of word file while test 1 matches outside of it. Nonetheless, they all have identity match greater than 50%. I am a computer scientist having great experience in data science. For me it is all just data and I can apply my data science experience to any domain even if I have little domain knowledge. Once we talk and discuss, I will get to know more about your requirements and can work to provide exactly what you need if this is not what you were looking for. Thanks.
$1,000 USD in 7 days
5.0 (1 review)
0.8
0.8
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I specialize in data processing and have worked on numerous projects such as sequence alignment in a high-throughput way. With my expertise in sequence alignment, I am confident that I can provide you with results that meet your needs. I have the necessary skills and experience to complete this project including identifying test sequences that have at least one stretch of 15-18 bp with at least 50% identity to the benchmark. I'm ready to tackle diverse projects with precision and creativity. If you're interested in working together on this project please let me know so we can begin discussing further?
$500 USD in 2 days
0.0 (0 reviews)
0.0
0.0
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HI SIR,,MAM I am full expert in data entry I read your all requirements I can replace your head with another picture and that will look 100% real I will give you best work on your time and budget I m new on freelancer but not this job trust me sir, mam.. just give me one chance to show my talent thanks waiting for your response....
$250 USD in 7 days
0.0 (0 reviews)
0.0
0.0
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HI SIR,,MAM I am full expert in data entry I read your all requirements I can replace your head with another picture and that will look 100% real I will give you best work on your time and budget I m new on freelancer but not this job trust me sir, mam.. just give me one chance to show my talent thanks waiting for your response....
$250 USD in 7 days
0.0 (0 reviews)
0.0
0.0
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I have software experiences in Data entry Excel Microsoft Word files PowerPoint Open Office Access Again, I have an addiction of keeping customers and clients satisfied. Skilled with 5 years experience with the high rate reviews from the appeased clients. Seek for new experiences with the exquisite will of progressing, learning and gathering knowledge related to this sector. I have possessed a high portfolio with high demands and better understanding in ‘analytically problem solving methods.’ Also, I have proficiency in handling complex situations and shortcomings by generating diplomatic techniques. thankyou sir!.....
$500 USD in 1 day
0.0 (0 reviews)
0.0
0.0
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Hello, Greetings! I hope you are doing great. I am writing to express my enthusiasm for the opportunity to collaborate on this project. With over 12 years of dedicated experience in the field, I bring a wealth of expertise to ensure the success of your endeavor. Here's why I am the perfect fit for your project: 1. With a robust portfolio spanning diverse projects, I have consistently delivered high-quality results, earning the trust of clients over the years. 2. My proficiency extends across various aspects of this field. This enables me to provide a holistic approach to your project needs. 3. I pride myself on staying ahead of industry trends and leveraging cutting-edge technologies to bring innovative solutions to the table, ensuring your project remains ahead of the curve. Now, to better understand your vision and requirements, could you please provide insights into the following: 1. What is your preferred timeline for project completion, and are there any critical milestones? Understanding your objectives will help me tailor my approach to meet your expectations effectively. 2. Could you share the primary goals you aim to achieve with this project? 3. Are there specific collaboration or communication preferences you have for seamless project coordination? I am eager to discuss how my skills align with your project requirements in more detail. Looking forward to the possibility of working together and achieving remarkable results. Warm regards, Manish Sharma
$500 USD in 7 days
0.0 (0 reviews)
0.0
0.0
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Dear Hiring Manager, As a newcomer in the field of data entry, I am very enthusiastic about the opportunity to join the Data Entry project at your company. Although I am a newcomer, I have accumulated basic knowledge and possess accurate and swift data entry skills. I believe that my high level of concentration and attention to detail in handling data will enable me to perform the job efficiently. Additionally, I am always seeking opportunities to self-improve and learn from a professional working environment such as yours. Ready to learn and work diligently, I hope to contribute to your project. I look forward to the possibility of meeting and discussing more about my skills. Thank you for considering my application. Best regards, Quan
$250 USD in 3 days
0.0 (0 reviews)
0.0
0.0
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The project description is poorly formulated. I am familiar with aligners and can give you a customized Excel workbook that performs pairwise alignment and gives you the maximum stretch and identity found in your sequences. I can also write the same codes in C++, Python, or R, depending on what environment is easiest for you.
$500 USD in 7 days
0.0 (0 reviews)
0.0
0.0
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Greetings, I am a biotechnologist with a Ph.D. in Biological Sciences, specialized in Bioinformatics and data science. I am enthusiastic about contributing to your project and am prepared to deliver a fully commented and automated pipeline tailored to your specific requirements. I have successfully aligned your test cases, identifying regions of high identity (>50%) in each instance, while excluding other sequences with similar identity levels outside the region of interest. Alignments for Test Sequence 1: benchmark 199 - 216: Benchmark: CAGTGATAAGATATGTG Test: CACTGTTAGGAAATCTG Consensus: CANTGNTANGANATNTG Identity: 70.59% benchmark 406 - 423: Benchmark: CAAGTACGCGGATCCTG Test: CATGTTCGAGGTTCATG Consensus: CANGTNCGNGGNTCNTG Identity: 70.59% Alignments for Test Sequence 2: benchmark 199 - 216: Benchmark: CAGTGATAAGATATGTG Test: CAGTGTTAAGAAATGTG Consensus: CAGTGNTAAGANATGTG Length: 17 Identity: 88.24% benchmark 406 - 423: Benchmark: CAAGTACGCGGATCCTG Test: CAAGTTCGCGGTTCCTG Consensus: CAAGTNCGCGGNTCCTG Identity: 88.24% Alignments for Test Sequence 3: benchmark 199 - 216: Benchmark: CAGTGATAAGATATGTG Test: CAGTGTTAAGAAATGTG Consensus: CAGTGNTAAGANATGTG Identity: 88.24% benchmark 406 - 423: Benchmark: CAAGTACGCGGATCCTG Test: CATGTTCGAGGTTCATG Consensus: CANGTNCGNGGNTCNTG Identity: 70.59% I'm eager to dive into this project and make it a success. I hope you find my skills and dedication aligning with your project needs.
$350 USD in 7 days
0.0 (0 reviews)
0.0
0.0

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